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Plasmid copy from coverage

Additionally to analyzing quality of samples, you can also use coverage to predict copy number of extrachromosomal elements. If chromosomal templates and extrachromosomal templates are in a 1:1 relationship, you expect equal distribution of coverage.

Sequencing read analysis

Ever wondered how to assess the quality of your NGS data? But get confused about all the fancy tools? Well how about i told you you can assess a lot of problems before even looking at the mapping yet, just by extracting some basic coverage data from BAM files, and extracting the number of reads per sample.